Part:BBa_K496004:Design

Reporter of Salmonella secretion signal with NLS, GFP, etc

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1205
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1144
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 979
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 2517
  Illegal SapI site found at 961
  

Design Notes

SlrP signal source is Salmonella enterica subsp. enterica serovar Typhimurium str. LT2. Amplified b y PCR.

Source

SlrP amplified with primer with RBS and flanking restriction site.